Keywords :
Russulaceae; Mushroom; Classification; DNA Barcoding; Phylogenetictree
บทคัดย่อ :
Lactarius and Russula are ectomycorrhizal fungi important for forest ecosystems, and many of which are edible mushrooms. In Basidiomycota, these genera are very diverse and possess complicated characters resulting in a number of under described species, especially in South East Asia. Molecular method, i.e. DNA barcoding, has been increasingly used to assist resolving taxonomical problems of mushrooms, especially in these two genera. Here, we collected nine specimens from Northeastern Thailand and identified by both morphology and DNA barcoding. We found that eight of the nine specimens were classified into Russula and the other one was classified as Lactarius. Of the nine specimens, seven specimens could be identified at the species level by morphology and the result was concordant with the barcoding. The other two specimens, however, could not be identified morphologically because of several unclear phenotypic characters. The barcoding results of these two specimens were also confusing because their sequences matched with several species from the database with equal percentage of identities. Our study provides new sequences of the described species to the database as references for the molecular identification of the mushrooms in the genera Lactarius and Russula.
เอกสารอ้างอิง :
Kaewgrajang, T., Kaewjunsri, S., Jannual, N. and Nipitwattanaphon, M., 2020. Morphology and molecular identification of some Lactarius and Russula species. Genomics and Genetics, 13(2&3), pp.44-58.
