Keywords :
Methanotrophic Bacteria
Tropical Upland Soils
บทคัดย่อ :
Three upland soils from Thailand, a natural forest, a 16-year-old reforested site, and an agricultural field,
were studied with regard to methane uptake and the community composition of methanotrophic bacteria (MB).
The methane uptake rates were similar to rates described previously for forest and farmland soils of the
temperate zone. The rates were lower at the agricultural site than at the native forest and reforested sites. The
sites also differed in the MB community composition, which was characterized by denaturing gradient gel
electrophoresis (DGGE) of pmoA gene fragments (coding for a subunit of particulate methane monooxygenase)
that were PCR amplified from total soil DNA extracts. Cluster analysis based on the DGGE banding patterns
indicated that the MB communities at the forested and reforested sites were similar to each other but different
from that at the farmland site. Sequence analysis of excised DGGE bands indicated that Methylobacter spp. and
Methylocystis spp. were present. Sequences of the forest soil cluster or upland soil cluster , which is
postulated to represent organisms involved in atmospheric methane consumption in diverse soils, were
detected only in samples from the native forest and reforested sites. Additional sequences that may represent
uncultivated groups of MB in the Gammaproteobacteria were also detected.
เอกสารอ้างอิง :
Knief, C., Vanitchung, S., Harvey, N. W., Conrad, R., Dunfield, P. F., & Chidthaisong, A. (2005). Diversity of methanotrophic bacteria in tropical upland soils under different land uses. Applied and Environmental Microbiology, 71(7), 3826-3831.
