Keywords :
Streptomyces,
soil,
Xylanase activity.
บทคัดย่อ :
A novel actinomycete, strain SR2-123T
, belonging to the genus Streptomyces, was isolated from a soil sample collected from
the Sakaerat Environmental Research Station, Thailand Institute of Scientific and Technological Research, Nakhon Ratchasima
Province, Thailand. The taxonomic position of the strain was characterized using a polyphasic study. Strain SR2-123T
contained
LL-diaminopimelic acid, glucose, mannose and ribose in whole-cell hydrolysates. The N-acyl type of muramic acid was acetyl.
Menaquinones were MK-9(H6), MK-9(H8) and MK-9(H4). The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-
C16 : 0, anteiso-C17 : 0 and iso-C17 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine,
phosphatidylglycerol, phosphatidylinositol, an unknown phospholipid, unknown glycolipids, an unknown aminophospholipid,
unknown lipids and an unknown aminolipid. The DNA G+C content was 74.8 mol%. The strain was closely related to
Streptomyces coeruleorubidus JCM 4359T
(98.5 %), Streptomyces flavofungini JCM 4753T
(98.5 %), Streptomyces coerulescens
NBRC 12758T
(98. 5 %) and Streptomyces alboflavus JCM 4615T
(98.4 %), based on 16S rRNA gene sequence similarities. The
novel strain exhibited low DNA-DNA relatedness values with the type strains (11.4-25.0 %) of closely related species. On the
basis of phenotypic and genotypic characteristics, strain SR2-123T
could be distinguished from closely related species of the
genus Streptomyces and represents a novel species of the genus Streptomyces for which the name Streptomyces xylanilyticus
sp. nov. is proposed. The type strain is SR2-123T
(=TISTR 2493T
=KCTC 39909T
).
เอกสารอ้างอิง :
Moonmangmee, D., Kanchanasin, P., Phongsopitanun, W., Tanasupawat, S., & Moonmangmee, S. (2017). Streptomyces xylanilyticus sp. nov., isolated from soil. International Journal of Systematic and Evolutionary Microbiology, 67(10), 4189-4194.
