Keywords :
AgNPs,
Antimicrobial Activity,
Optimization,
Pseudomonas aeruginosa,
Streptomyces sp. SSUT88A
บทคัดย่อ :
The green method has become an environmentally safe and valuable alternative to
synthesizing silver nanoparticles (AgNPs). The AgNPs has been applied as antimicrobial agents,
which their toxicity depends on several variables that generate different ability to inhibit
pathogenic bacteria. Therefore, the optimization of AgNPs synthesis plays an important role in
providing good antimicrobial activity. In this study, the synthesis of AgNPs was carried out with
three different parameters: time of incubation, pH, and temperature to inhibit Pseudomonas
aeruginosa growth using cell-free supernatant of Streptomyces sp. SSUT88A. The UV-Vis
spectroscopy and antimicrobial activity were measured to obtain the optimum condition for each
condition. The 74.12 nm in the spherical shape of AgNPs were optimized at 37 C, under pH 7
for five days. The synthesized AgNPs exhibited antimicrobial activity against P. aeruginosa
1287 and multidrug-resistance P. aeruginosa N90PS.
เอกสารอ้างอิง :
Rosyidah, A., Nantapong, N., Chudapongse, N., Weeranantanapan, O. and Limphirat, W., 2021, December. Optimization of silver nanoparticles synthesis by the green method using Streptomyces sp. SSUT88A and their antimicrobial activity against Pseudomonas aeruginosa. In IOP Conference Series: Earth and Environmental Science (Vol. 948, No. 1, p. 012085). IOP Publishing.
