Keywords :
16S rRNA; Termes propinquus; Higher Termite; Gut Bacteria; Cellulase; Bacillus amyloliquefacien sub sp.; Plantarum Strain; FZB42; B. methylotrophicus; Strain; CBMB205
บทคัดย่อ :
Termites are social insect in terrestrial ecosystems and feed on dead plant materials such as wood that composed of important constituents: cellulose, hemicellulose and lignin which are difficult to decompose. Termite gut is habitat of highly bacterial diversity that can degrade cellulose and other plant components into smaller molecules of nutrients. In this study, 2.56 x 106 CFU/g of gut content were obtained from the gut of wood/soil interface-feeding higher termite, Termes propinquus (Termitinae) which collected from Sakaerat Environmental Research Station, Wang Nam Kiew, Nakhon Ratchasima, Thailand. Fourteen different cultivable bacteria were characterized with their morphologies including Gram-positive rod, Gram-positive filamentous and Gram-negative cocci bacteria. Many bacteria showed their growth under various conditions including extreme conditions of pH and temperatures. The gut bacteria showed enzymatic activities on the nutrient agar supplemented with various substrates for cellulase, pectinase, xylanase, amylase, lipase, protease and gelatinase at room temperature. Among all bacteria, 3, 8, 2, 7, 6, 5 and 8 isolates were characterized to cellulolytic, pectinolytic, xylanolytic, amylolytic, lipolytic, proteolytic and gelatinolytic bacteria, respectively. Bacterial isolates Tp-6-2 and Tp-8-1 showed high cellulase activity on Berg s agar supplemented with carboxymethyl cellulose (CMC).Their cellulase activities under extreme conditions of pH and temperatures were measured to determine their optimum conditons. Molecular identification based on 16S rRNA gene sequence revealed that the isolates Tp-6-2 and Tp-8-1 were closely related to Bacillus amyloliquefacien subsp. plantarum strain FZB42 (99% nucleotide identity) and B. methylotrophicus strain CBMB205 (99% nucleotide identity), respectively. Phylogenetic tree revealed relationship of the 16S rRNA gene sequences among the isolated bacteria and Bacillus group. This is the first report on isolation of gut bacteria from wood/soil interface-feeding higher termite, T. propinquus. Our results indicated that termite guts are attractive source for the study of bacteria and bacterial enzymes benefit for biotechnology.
เอกสารอ้างอิง :
Sakolvaree, J., & Deevong, P. (2016). Isolation and Characterization of Cellulase Producing Bacteria from the Gut of a Higher Termite, Termes propinquus. Burapha University International Conference
